Data Trawler - Project details

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Project details

Title: Flow cytometric classification of the phytoplankton community across Australia’s top end
Id: 2597
Investigator(s): Allison McInnes
Queensland University of Technology [details]

Description: Phytoplankton are a diverse group of autotrophic organisms that range in size, shape, activity, and role in marine ecosystems. Understanding the composition of the phytoplankton community offers insight into the functioning of these systems. Phytoplankton Class can be determined by pigment composition, analysis of which offers compositional understanding of these important ecosystem engineers; hence much development has been done in analysis of pigments. Current methods require large volumes of water and are amalgamations of the total community pigments. The proposed research will utilise the spectral signature capability of the Cytek Aurora to classify phytoplankton using their pigment composition at the single cell level using small volumes.
  • Due to COVID-19 participant restrictions, the Cytek Aurora is not able to be installed onboard
  • A modified sampling program has been included to try and facilitate this work, with samples collected to be freighted back to Brisbane upon arrival in Darwin.
  • Years: 2021

    List of surveys that this project was on.

    Use [details] link to view survey details (map, reports, metadata etc) including links to download data.

    Survey InvestigatorDescription
    IN2021_T01

    [details]
    V. Puigcorbe (Edith Cowan University) RV Investigator transit voyage between Brisbane and Darwin: in2021_T01, with the following supplementary and piggyback projects:
  • Microplastics in the food chain: impact on the microbial and planktonic organisms
  • Linking the Biological Carbon Pump flux to microbial colonisation of sinking particles in the Coral Sea
  • Dinoflagellates & broader planktonic assemblage observation
  • BGC-Argo Float Deployment
  • Cosmic Ray Measurements
  • Carbon Sampling
  • Flow cytometric classification of the phytoplankton community across Australia’s top end
    The primary objective of voyage IN2021_T01 is movement of RV Investigator from Brisbane to Darwin in preparation for IN2021_V04. Up to 72 hours of the transit voyage have been allocated to conduct scientific operations which will consist of a minimum of 12hrs devoted to the Supplementary Project: Microplastics in the food chain: impact on the microbial and planktonic organisms. The remaining time can be available for multiple Piggyback Projects. Some opportunistic mapping has been prepared should we be ahead of schedule.
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